ang1 7 (MedChemExpress)
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ang1 7
Ang1 7, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ang1+7/Talfirastide/pmc12749397-25-13-18
Average 95 stars, based on 26 article reviews
Ang1 7, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ang1+7/Talfirastide/pmc12749397-25-13-18
Average 95 stars, based on 26 article reviews
ang1 7 - by Bioz Stars,
2026-09
95/100 stars
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1) Product Images from "Ginsenoside Rg1 alleviates HG-induced autophagy of retinal microvascular endothelial cells by activating ACE2/Ang1-7/Mas in vitro"
Article Title: Ginsenoside Rg1 alleviates HG-induced autophagy of retinal microvascular endothelial cells by activating ACE2/Ang1-7/Mas in vitro
Journal: Scientific Reports
doi: 10.1038/s41598-025-29344-0
Figure Legend Snippet: Effects of Ang1-7 on viability, the ACE2/Ang1-7/Mas axis and autophagy in HG-induced HRMECs. ( A ) HRMECs were subjected to 48-h incubation with 5.5, 5.5 mM NG + 24.5 mannitol and 30 mM glucose (NG, MA and HG groups), respectively, followed by 100 nmol/L Ang1-7 treatment (HG + Ang1-7 group). MTT assay was used to detect cell viability in the three groups. ( B and C ) Cell apoptosis was assessed with the flow cytometry experiment. ( D – F ) Western blot was performed to measure protein expressions of ACE2 and Mas in the three groups. ( G ) ELISA was utilized to detect Ang1-7 level in the three groups. ( H – J ) Western blot was applied to measure protein expressions of LC3II/LC3I and P62 in the three groups. ( K ) and ( L ) Microstructural detection of autophagosomes by transmission electron microscopy (scale bar = 200 µm) in the three groups, arrow: autophagosomes. Data are shown as mean ± standard deviation (SD) (n = 3 biological replicates). GAPDH was used as the loading control. *** P < 0.001, versus NG; +++ P < 0.001, versus HG. HRMECs, human retinal microvascular endothelial cells; NG, normal glucose; HG, high glucose; Ang1-7, angiotensin 1–7; ACE2, angiotensin-converting enzyme 2; MTT, methylthiazolyldiphenyl-tetrazolium bromide; ELISA, Enzyme-linked immunosorbent assay.
Techniques Used: Incubation, MTT Assay, Flow Cytometry, Western Blot, Enzyme-linked Immunosorbent Assay, Transmission Assay, Electron Microscopy, Standard Deviation, Control
Figure Legend Snippet: The protective role of Rg1 in HG-induced HRMECs through the ACE2/Ang1-7/Mas axis. ( A ) HRMECs were subjected to 48-h incubation with 5.5 and 30 mM glucose (NG and HG groups), respectively, followed by treatments with 1 μmol/L A779 (a Mas receptor antagonist) and/or 10 μM Rg1 (HG + A779, HG + Rg1 and HG + Rg1 + A779 groups). MTT assay was used to detect cell viability in the five groups. ( B ) and ( C ) Cell apoptosis was assessed with the flow cytometry experiment. ( D – F ) Western blot was performed to measure protein expressions of ACE2 and Mas in the five groups. ( G ) ELISA was utilized to detect Ang1-7 level in the five groups. ( H ) and ( I ) Western blot was conducted to measure protein expressions of p-eNOS and eNOS in the five groups. ( J ) and ( K ) ELISA was used to detect the levels of TNF-α, and IL-6. ( L – N ) Western blot was conducted to measure protein expressions of LC3II/LC3I and P62 in the five groups. ( O ) and ( P ) Microstructural detection of autophagosomes by transmission electron microscopy (scale bar = 200 µm) in the five groups, arrow: autophagosomes. Data are shown as mean ± standard deviation (n = 3 biological replicates). GAPDH was used as the loading control. *** P < 0.001, versus NG; + P < 0.05, ++ P < 0.01, +++ P < 0.001, versus HG; # P < 0.05, ### P < 0.001, versus HG + Rg1; ^^^ P < 0.001, versus HG + A779. Rg1, ginsenoside Rg1.
Techniques Used: Incubation, MTT Assay, Flow Cytometry, Western Blot, Enzyme-linked Immunosorbent Assay, Transmission Assay, Electron Microscopy, Standard Deviation, Control
Figure Legend Snippet: Involvement of PI3K/Akt pathway in the mitigating effect of Rg1 on HG-caused dysfunctional HRMECs. ( A ) HRMECs were subjected to 48-h incubation with 30 mM glucose (HG group), followed by treatments with 10 μM Rg1 and/or 50 μM LY294002 (HG + Rg1, HG + LY294002 and HG + Rg1 + LY294002 groups). MTT assay was used to detect cell viability in the four groups. ( B and C ) Cell apoptosis was assessed with the flow cytometry experiment. ( D – H ) Western blot was performed to measure protein expressions of p-Akt/Akt, ACE2 and Mas in the four groups. ( I ) ELISA was utilized to detect Ang1-7 level in the four groups. ( J – L ) Western blot was conducted to measure protein expressions of LC3II/LC3I and P62 in the four groups. ( M ) and ( N ) Microstructural detection of autophagosomes by transmission electron microscopy (scale bar = 200 µm) in the five groups, arrow: autophagosomes. Data are shown as mean ± standard deviation (n = 3 biological replicates). GAPDH was used as the loading control. + P < 0.05, +++ P < 0.001, versus HG; # P < 0.05, ### P < 0.001, versus HG + LY294002; ^^ P < 0.01, ^^^ P < 0.001, versus HG + Rg1. Akt, protein kinase B; p-Akt, phosphorylated Akt.
Techniques Used: Incubation, MTT Assay, Flow Cytometry, Western Blot, Enzyme-linked Immunosorbent Assay, Transmission Assay, Electron Microscopy, Standard Deviation, Control
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